Sentence examples for mouse embryonic feeder from inspiring English sources

Less pronounced intercellular communication was also observed between human embryonic stem cells and mouse embryonic feeder cells [17].

hESCs were maintained on irradiated mouse embryonic feeder cells derived from C57BL/6 mice (E12.5 E13.5) in knockout-DMEM media as described [4] (Invitrogen).

D3 mouse ESCs were grown on mouse embryonic feeder (MEF) layer in ESC medium [DMEM containing 15% fetal bovine serum (FBS), β-mercaptoethanol, non-essential amino acids, glutamine (all Invitrogen) and 1,000 U/ml leukemia inhibitory factor (Chemicon)].

The Ainv15 ES cell line was maintained on irradiated mouse embryonic feeder (MEF) cells in Dulbeccós modified Eagle medium (DMEM) (Gibco-BRL, United Kingdom) supplemented with 15% fetal calf serum (FCS) (Boehringer, Germany), 1.5×10−4 M monothioglycerol (MTG) (Sigma, Germany) and LIF (Chemicon, Ca, USA).

Wild type ES cell lines R1, J1, D3 and B6 were cultured on mitotically inactivated mouse embryonic feeder (MEF) cells in Dulbecco's Modified Eagle medium (DMEM, Hyclone) with 10% ES quality foetal bovine serum (FBS; Hyclone), 1 mM sodium pyruvate (Gibco), Penicillin (100units/ml)/Streptomycin (100 µg/ml) (Gibco), 5.5×10−2 mM β-mercaptoethanol, (Calbiochem) and 103 U/ml LIF (Chemicon).

GS cells were established from P7 testes and maintained on mitotically inactivated mouse embryonic feeder cells as previously described [ 40].

Cells were passaged with 0.05% trypsin-EDTA (Gibco) every third or fourth day onto freshly seeded (25,000/cm2) mitotically inactivated mouse embryonic feeder-cells (MEFs) (Sahlgrenska Akademin Experimental Biomedicine University of Gothenburg) at a density of 12,000 cells/cm2 for Hues-3 (subclone 52) and 30,000 cells/cm2 for Hues-15.

The hESC were maintained in the undifferentiated state on mitomycin-C-treated mouse embryonic feeders (MEF) obtained from Millipore (Billerica, MA, http://www.millipore.com).com

We have used mouse embryonic fibroblast feeder cells transfected with the neomycin resistance gene in order to detect feeder cell contaminations.

ES cells were thawed on plates with a mitomycin C-treated mouse embryonic fibroblast feeder layer and then grown and expanded on 0.1% gelatin-coated plates (without feeder cells) in medium containing LIF for subsequent experiments.

In vitro, the ES cells differentiated when cultured in the absence of mouse embryonic fibroblast feeder layers, both in the presence and absence of human leukemia inhibitory factor (LIF) (Fig. 1).