Boroviak T, Loos R, Bertone P, Smith A, Nichols J, 2014, RNA sequencing of mouse embryonic day 3.5 blastocyst inner cell mass cells, http://www.ebi.ac.uk/ena/data/view/ERP005749, Publicly available at the EBI European Nucleotide Archive (Accession no: ERP005749).
In undifferentiated female and male mouse embryonic stem cells and embryonic blastocysts, the F M ratio of X-linked genes is 1.61.
This raises the possibility that a physiological function of Mcl-1 may be linked to intracellular Ca2+ trafficking to permit blastocyst adhesion during mouse embryonic development.
The blastocyst complementation system was first reported by Chen et al. by implanting normal mouse embryonic stem cells (mESCs) into the blastocysts derived from Rag2−/− mice to generate T and B lymphocyte lineages [ 8].
H3f3bFl/WT mouse embryonic stem cells were injected into blastocysts to produce chimeras, which were then bred with wildtype mice.
Injection into another mouse's blastocyst is the standard test for mouse embryonic stem cells.
In order to better understand the functions of PDCD2 in mammalian development, we have examined PDCD2 activity in mouse blastocyst embryos, as well as in mouse embryonic stem cells (ESCs) and embryonic fibroblasts (MEFs).
Our results demonstrate an important function of PDCD2 in proliferation, particularly for the G1/S phase transition of the cell cycle in mouse embryonic fibroblasts (MEFs) and ESCs, as well as in mouse blastocyst embryos.
Mouse embryonic stem (ES) cells are derived from the ICM of the blastocyst.
If cultured mouse embryonic stem cells are injected into an early mouse embryo at the blastocyst stage, they will become integrated into the embryo and produce cells that differentiate into most or all of the tissue types that subsequently develop.
