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Now Lee's group appears to have found a winner: a gene for a growth factor that appears to be switched on only in mouse embryo tissue destined to become skeletal muscle.
In intial studies on the distribution of nestin in mouse embryo tissue we detected a coincident strong accumulation of GR in nestin positive cells.
Here we show that a strong nestin expression in mouse embryo tissue coincides with a strong accumulation of the glucocorticoid receptor (GR), a key regulator of growth and differentiation in embryonic development.
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The vaccine for yellow fever virus, a pathogen that is maintained in African and South American primates under natural conditions, was created via passage through Asian macaques, mouse embryo tissues and chick embryo tissues (Fig. 1b).
The filters from normal human and mouse embryo tissues were purchased from Clontech (Palo Alto, CA, USA).
Mouse embryo multiple-tissue northern blot and mouse adult multiple-tissue northern blot filters were purchased from Clontech Laboratories (Palo Alto, CA).
Mouse embryos, placental tissues, fetal tissues, and adult tissues were prepared as described previously.,, To use DNA polymorphisms for allele discrimination, reciprocal F1 hybrids were obtained by crossing C57BL/6N (B6; Clea Japan) and JF1/Msf (JF National Institute of Geneticss) mice.
The expression of Sprouty4 is not affected in Hes7-null mutant mouse embryos in tissues other than the PSM, although the metameric pattern in the somites is faded (Figure 3A).
Total RNA from cell cultures, mouse embryos and tissues was extracted by Trizol reagent (Invitrogen) and treated with DNase I before converted to cDNA by using SuperScript II reverse transcriptase (Invitrogen) with oligo d(T) primer following the manufacturer's instruction.
For mT and mG detection, mouse embryos or tissues were directly imaged under a Leica MZFLIII fluorescence dissecting microscope.
In the mouse embryo, visceral endoderm, the tissue homologous to the chick anterior endoderm, is required for terminal differentiation of cardiomyocytes [5].
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