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The EURExpress project aims to build a transcriptome-wide atlas for the developing mouse embryo established by RNA ISH.
For example, the EUREXPress project (Eurexpress, 2009) has built a transcriptome-wide atlas for the developing mouse embryo established by RNA ISH; it has so far collected more than 18 000 genes at one development stage of wild-type murine embryos and has curated 4 TB of images.
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Torres-Padilla, M. E. et al. The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation.
Primary MN cultures were established from E13.5 C57BL/6J mouse embryos using established methods [34].
In the mouse embryo, methylation of histone H3 establishes an epigenetic asymmetry and is predominant in the maternal pronucleus.
In the mouse embryo, the L-R axis is established at approximately 8.25 days of development.
In mouse embryos it has been established that GATA6-stimulated fibroblast growth factor (FGF) signalling via the extracellular signal-regulated protein kinase (ERK) is responsible for NANOG repression and thereby the formation of primitive endoderm [ 2, 10- 12].
This analysis reveals that Dmrt2 is not important to establish LPM laterality in the mouse embryo.
These findings are consistent with prior studies demonstrating that the node is not essential for establishing anterior posterior polarity of the developing mouse embryo (Davidson et al., 1999).
We discuss how the asymmetric localization and segregation of Cdx2 transcripts could contribute to multiple mechanisms that establish different cell fates in the mouse embryo.
The impasse was gradually resolved by first using an empirical approach that relies on the stochastic differentiation of mouse embryos or ESCs to establish lineage-restricted cell lines with endothelial-like potential.
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