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Of the gRNA-2 group, we obtained 3 (27.3%) biallelic mutant founder mice (P3, P6, P11), in line with the mouse embryo data (Fig. 2E and Table 1).
In contrast to experiments exhibiting two or more periods, which are well suited to Fourier analysis and related methods (see, e.g.,[30]), the mouse embryo data in this paper represents only a single period.
In the application to the mouse embryo data, we use what is sometimes called the bounded variation, <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0002856.e007.PNG" class= inline-graphic"/>, where πi is the rank of fi in the sorted order (see Figure 6 left for an example).
Our preliminary data indicate, however, that combining Ifih1 with Adar1 does not rescue the lethality of the Adar1 mutant mouse embryo (data not shown), suggesting that MDA5 is not the sole mediator of the Adar1 mutant phenotype.
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We also looked at p300 binding sites in forebrain, midbrain and limbs of E11 mouse embryos (data from [39]), but none of our identified enhancers overlapped with a peak of p300 binding in these tissues.
This distribution, particularly near the eyes and in the pectoral fin buds, is consistent with that seen for CPA6 mRNA in the mouse embryo [1] unpublished data), further confirming a molecular and functional homology.
For example, the mouse embryo gene expression data may come from different data sources.
Motivation: Single-cell experiments of cells from the early mouse embryo yield gene expression data for different developmental stages from zygote to blastocyst.
In contrast to Bmp4-deficient kidney rudiments (Figure 1A, 1B), no ectopic ureteric buds were observed in mouse embryos lacking Bmp7 (data not shown).
We then took advantage of similar analysis in telencephalon microdissected into the cortex and ganglionic eminence (GE) in Sey/Sey mouse embryos [17] for data comparison.
Johnson and his group wrote an algorithm that distinguishes and visualizes various organs and structures in the mouse embryo based on the microCT scan data.
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