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However, even studies of mouse embryos lack analysis of the earliest stages of cloaca patterning and morphogenesis.
PCR analysis of mouse embryo fibroblasts has shown both splice variants to be present in these cells; however, to our knowledge, no analysis of the relative expression levels of these two transcripts in human cell lines or tissues has yet been reported.
In mouse embryo, long-term in vivo analysis of cell behaviours and movements is difficult because of the development in utero and the impossibility of long-term culture.
No lens progenitors are formed in the pre-placodal region surrounding the neural plate of the Pax6−/− mouse embryo [42], thus precluding such analysis.
MPSS analysis of mouse embryo small RNA discovered over 60 potential novel microRNAs, some of which are rodent specific [ 11].
Transcriptome analysis in mouse embryo fibroblasts treated with TGFβ revealed that Smad3 appeared to be the dominant transcriptional regulator downstream of the TGFβ receptor, and that Smad2 functioned primarily in a transmodulatory fashion [ 11].
To investigate whether changes in Hif1α mRNA expression are accompanied by variations at the protein level and to analyse Hif1α localisation in 8.5 dpc mouse embryos, an immunofluorescence analysis was performed on paraffin sections.
This continuity has been confirmed in both chick and mouse embryos through the analysis of molecular markers whose expression is continuous from the primitive streak cells into subpopulations of cells in the tailbud region (Gofflot et al., 1997; Knezevic et al., 1998).
The inability of study-level meta-analysis to overcome partial consistency on a limited number of studies was also observed in the meta-analysis of mouse embryo studies [ 3].
This main finding demonstrates the neuromesodermal bipotency of in vitro derived NMP at the single cell level, recapitulating the behaviour of NMPs as identified by retrospective clonal analysis in the mouse embryo in Tzouanacou et al. 2009.
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