The data in Figure 2, with other reports of integrin subunit expression, indicate that mouse eggs express three of eight β subunits and eight of 18 α subunits (Itgb1, Itgb3, Itgb5, Itga1, Itga2, Itga3, Itga5, Itga6, Itga8, Itga9, Itgav).
Analysis of integrin subunit expression indicates that mouse eggs could express at least ten different integrins, five in the RGD-binding family, two in the laminin-binding family, two in the collagen-binding family, and ITGA9-ITGB1.
Mouse and human eggs express ITGA9 [10], and ITGA4 expression has been observed in bovine, hamster, pig, and human eggs [12], [13], [14], [15].
Thus, mouse eggs could express at least ten different integrins, with five in the family of integrins that bind RGD tripeptide in ligands (e.g., fibronectin, vitronectin), two in the laminin-binding family, two in the collagen-binding family, and ITGA9-ITGB1, whicancan interact with a variety of ligands (Fig. 6) [2], [2].
Proportion of eggs expressing Venus in the head region at each embryonic stage (n ≥9 animals).
Fifth column: time points from movies of eggs expressing mCherry::PGL-3.
However, microinjection of wild-type human PLCζ protein into mouse eggs that were expressing these infertility-linked PLCζ mutants effectively rescued the failure of egg activation by inducing a normal pattern of Ca2+ oscillations, leading to successful early embryo development up to the blastocyst stage (see Fig. 6).
The egg's extracellular coat is composed of several glycoproteins; human eggs express ZP1, ZP2, ZP3 and ZP4.
From our previous calibration of luciferase expressed in mouse eggs [ 10], we can determine that the luminescence from single CHO cells expressing PLCζ luc (~10 cps) corresponds to >100 fg of the fusion protein.
Ae2 is the primary AE family HCO3−/Cl− exchanger expressed in mouse eggs [28], [29].
