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RNA was prepared from the skin of mouse ears using TRIzol reagent (Invitrogen, Carlsbad, CA) and reverse transcribed according to a published protocol [44].
Positive transgenic founder mice were identified by PCR on DNA isolated from mouse ears using human specific primers (for primer sequences see Additional file 1: Table S1).
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DPOAEs were recorded first using two TDT MF-1 speakers and an ER-10B+ microphone (Etymotic, Elk Grove Village, IL) coupled to the mouse's ear using a modified pipette tip (a 10 µl pipette tip was trimmed such that length = 10 mm and the diameter at the tip opening = 2 mm).
To test the feasibility of measuring MRO2, the microvasculature in mouse ears was used as an analogue of retinal vessels because both objects are optically thin.
He wore a pink shirt and round glasses, and was talking to Arne Glimcher, his dealer, about the first time he'd used mouse ears in his work — on masks, in a performance piece that took place on West Forty-first Street, in 1965.
On the same day mice were treated with Lodamin (15 mg/kg orally) and ear thickness was then measured daily (3 measurements per ear using a caliper) for 12 days.
The mouse ear pinna was used as a living sample in the experiments with the goal to visualize and quantify the blood flow with microcirculatory tissue beds.
Mouse DNA was extracted from mouse ear clippings and purified using the DNeasy™ blood and tissue kit according to the manufacturer's protocol (Qiagen, Venlo, Netherlands).
Parasite numbers were determined using a standard curve made from mouse ears or lymph nodes spiked with 0, 200, 1000 and 5000 sporozoites.
By using a genetic technique, researchers increased the amount of NT3 production in mouse ears, allowing them to regain their hearing better than control mice, the International Business Times reports.
Madonna responded by tweeting an old picture of herself wearing Mickey Mouse ears, captioned: "From one mouse to another, I don't support drug use and I never have.
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