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Figure 9 shows the Mouse eFP Browser output for a muscle-specific protein, beta tropomyosin (Tpm2, XM_124262.1).
To begin, the form is prevented from being submitted with an improperly formed or absent AGI IDs (or RefSeq IDs in the case of the Mouse eFP Browser).
To illustrate the utility of the eFP Browser engine for displaying expression data from 55 tissues in mouse, we have also developed a Mouse eFP Browser, based on a data set generated by the Hughes laboratory [12].
We give examples of using the tool to present Arabidopsis gene expression data from the AtGenExpress Consortium (Arabidopsis eFP Browser), data for subcellular localization of Arabidopsis proteins (Cell eFP Browser), and mouse tissue atlas microarray data (Mouse eFP Browser).
In addition, we also provide examples of how we have used it to create a Cell eFP Browser for displaying protein subcellular localization data and a Mouse eFP Browser for displaying gene expression data from a mouse tissue atlas.
The Arabidopsis eFP Browser for exploring Arabidopsis microarray data encompassing more than 1000 microarray data sets produced by the AtGenExpress Consortium and from other labs is freely available to anyone with a web-browser and an internet connection at http://www.bar.utoronto.ca/, as is the Cell eFP Browser and the Mouse eFP Browser.
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In the case of the Arabidopsis, Cell and Mouse eFP Browsers, we have mirrored publicly-available microarray data from several sources – described in the Data Sources and subsequent two sections – in our Bio-Array Resource [10].
When the form is submitted, the Browser engine first converts the AGI ID or IDs to Affymetrix ATH1 GeneChip probe set identifier or identifiers via a lookup table, a copy of which was obtained from TAIR at www.arabidopsis.org (a probe set identifier or identifiers may also be entered – in this case no lookup takes place, as is currently the case with the Cell eFP and Mouse eFP Browsers).
Thanks also to Jeff Alls for his line drawings of Arabidopsis for the Arabidopsis Developmental Series, and Thea Ampofo for her line drawings for the Mouse and Cell eFP Browsers.
Besides, because OsLAP6/OsPKS1 had a strong and specific expression in rice flowers (Fig. 5), we therefore retrieved the electronic fluorescent pictograph (eFP) browser in the Bio-Analytic Resource for Plant Biology (BAR, http://bar.utoronto.ca).
The expression levels of the sRNA precursors, the sRNA target genes and the target mimics could be partially uncovered by visiting PmiRKB (plant microRNA knowledge base) (Meng et al., 2011a), mirEX (Arabidopsis pri-miRNA expression atlas) (Bielewicz et al., 2012), AVT (AtGenExpress visualization tool) (Kilian et al., 2007; Goda et al., 2008), and Arabidopsis eFP Browser (Winter et al., 2007).
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