Nanog transcripts and protein were detected in testes of mouse, dog, pig, and human.
We downloaded the protein and reference mRNA sequences for humans, mouse, dog, pig, cow, and horse from ENSEMBL.
We used protein and reference cDNA sequences of human, mouse, dog, pig, cow, horse, and dolphin from Ensembl [ 52] and the common minke whale from our results.
In the present study, we review the results in human and extend the examination of the evolutionary impact of heterochiasmy beyond primates to include four additional eutherian mammals (mouse, dog, pig, and sheep), a metatherian mammal (opossum), and a bird (chicken).
He adds that individuals with vivarium expertise are also in demand, as various animal models, such as mice, dogs, pigs, and monkeys, are needed for preclinical studies.
In the current study we describe NANOG expression in the testis and determine its expression pattern in mammalian species ranging from mouse, dog, and pig, to human.
For mouse, dog, and pig, total RNA was isolated from 50 100 mg testis sections with Trizol (Invitrogen), according to the manufacturer's protocol with an additional purification step immediately following phase separation.
Main variables: RRs, equilibrium GC (GC*), and observed GC contents were calculated using appropriately sized windows (described below) along each of the genomes of seven species: human, mouse, dog, sheep, pig, opossum, and chicken having available sex-specific genetic maps as well as genome assemblies.
We retrieved 457; 908; 845; and 1,301 OR sequences from human, mouse, dog, and pig, respectively, and combined them with cattle (1,071 putative OR genes from 1,423 putative genes minus 352 partial genes), then we aligned these 4,582 OR genes together using CLUSTALW [ 14].
An overview of rabbit, sheep, mouse, rat, dog, pig, monkey, and horse tendon and ligament models is given and the models are not only discussed in terms of suitability to represent the human situation, they are also compared to each other and with respect to their practicability.
The characterization of the majority of these genomic features will require functional assays in tissue culture or in vivo that will have to be carried out in animal models of CVD (e.g. mouse, rat, dog, pig).
