Contamination of specimens with mouse DNA will produce a false positive PCR result since mouse DNA contains endogenous MLV sequences that amplify by the diagnostic XMRV/MLV PCR assays.
The real-time qPCR assay was designed to estimate the percentage of human and mouse DNA contained in a xenograft or other mixed-DNA sample.
The main DNA sequence of chromocenters, major satellite repeat, is present on all autosomes, comprises ca. 10% of whole mouse DNA, contains ca. 50% of the CpG dinucleotides of the whole mouse genome [ 37], and was shown to bind MECP2 [ 1].
In the present study, we report a systematic analysis of putative tankyrase functions in mice deficient in either tankyrase 1 or tankyrase 2, as well as the phenotype resulting from deficiency in both tankyrase 1 and tankyrase 2. A tankyrase 1 gene targeting vector was constructed from an 11 kb SalI/KpnI fragment of mouse genomic DNA containing the first exon of tankyrase 1.
To address that concern, John Brigande, a developmental neurobiologist at Oregon Health and Science University in Portland, and colleagues injected embryonic mice with DNA containing several copies of Atoh1.
As previously described (Bao et al., 2009), Tg mice for Glud1 were generated by microinjecting oocytes from hyper-ovulating C57BL6/SJL hybrid mice with linearized DNA containing the cDNA for mouse Glud1 under the control of the promoter for neuron-specific enolase.
In an aged mouse model, prokaryotic DNA containing unmethylated cytidine phosphate guanosine (CpG) motifs yielded strong enhancement of the immunization effects of hepatitis B vaccine (Qin et al 2004).
A solution of plasmid DNA containing mouse BMP-4 (pMiw-BMP4) was injected into the gastrocnemius of BALB/cA mice, and electric pulses were applied through paired-needle electrodes inserted percutaneously.
But he wondered whether Bier's gene drive system could ferry the hefty 17,000 bases of DNA containing the mouse antibody genes.
The probe was prepared by labeling plasmid DNA containing a mouse Xist exon 1 sequence.
