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Other studies indicate that Smad4 is downregulated in metastatic prostate cancer tissues and knockdown of the Smad4 gene showed significantly enhanced metastatic potential to lung with PC-3 cells implanted in the renal capsule of an immunocompromised nude mouse (Ding et al, 2011).
Our results strongly suggest that mouse DING proteins are endogenous.
This demonstrates the high specificity of polyclonal antibody raised against mouse DING proteins and HPBP.
Because of the high sequence conservation within the DING protein family, these bands might also correspond to uncharacterized mouse DING proteins.
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Collectively, these findings demonstrate that we have successfully produced a reprogrammed adult mouse dsRed-iPS cell line that possesses the ability to produce cell types of all three germ layers, similar to embryonic stem cells.
Quantification of sialidase staining for human (c) and mouse (d) lung sections with ImageJ.
Therefore, it has not been possible to conclude whether the porcine D-loop ESTs correspond to any of the reported mouse D-loop ncRNAs.
Sequences with the identical V(D J recombination encoding ARGAY CDR3 peptide obtained from splenic B-1a sample from 4 month old specific pathogen free mouse, (B ) germ-free mouse (C ) and AID knockout mouse (D ) are listed.
The five expression profile fold values are shown with: A, severe AD in human; B, chronic spinal chord contusion in rat; C, chronic spinal chord contusion in mouse; D, SOD1(G93A) mutation late stage in mouse; E, prion disease in mouse.
With experiments in the dish and in live mice, Ding and colleagues showed that the resulting cells are fully pluripotent.
Recently, we found that the HMF could increase cellular ROS levels in primary skeletal muscle cells (Fu et al. 2016); however, no significant changes on oxidative stress signaling were detected in the HMF-exposed mice (Ding et al. 2014).
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