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After weaning, the mice of each genotype were fed a normal purified mouse diet containing 5% crude fat in form of LCT, corresponding to 12% of metabolizable energy as calculated with Atwater factors (ssniff® EF R/M Control, ssniff Spezialdiäten GmbH, Soest, Germany).
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(If a Western diet contains about 14.7 g of LA/day [ 23] the calories from LA are 6.6% of a 2000 calorie diet. The 10% corn oil mouse diet contained 10.9% of calories from LA).
Based on these observations, we fed R6/2 and WT mice diets containing 0.1% or 0.375% minocycline assuming that a 20 g mouse eats 4 g of food per day.
This mechanism was demonstrated in mice models by injecting viral genome in mice deficient in selenium as opposed to mice fed diet containing sufficient selenium.
Therefore, to test the effect in mice on a normal diet, the experiment had to be repeated by giving the mice a diet containing higher resveratrol so as to achieve similar plasma levels as was seen in the mice on a high calorie diet.
When Dr Renslo fed mice a diet containing the improved aminothiazoles, he found that the most promising of them accumulated in the brain in concentrations nearly 25 times higher than those required to clear prions from cultured cells.
Our experimental set-up is comparable to the one used by Vikstedt et al, as we also fed the mice a diet containing 0.25% cholesterol for 9 weeks, and we used an even slightly longer recovery period (9 weeks).
Demyelination was induced by feeding 8-week-old male C57BL/6 mice a diet containing 0.2% cuprizone (bis-cyclohexanone oxaldihydrazone; Sigma-Aldrich Inc., St . Louis MO) mixed into a ground standard rodent chow for 5 weeks.
We placed mice on diet containing curcumin at 0.6% for 6 weeks.
TCPOBOP-induced CAR activation prevented the increased hepatic and serum cholesterol caused by feeding mice a diet containing 1% cholesterol.
Briefly, one group underwent demyelination by feeding the mice a diet containing 0.3% cuprizone mixed into Teklad Global Rodent Diet Harlan Laboratoriess, Inc., Indianapolis, IN).
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