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We have evaluated this labeling strategy in three cell types: mouse melanoma cells (mMCs), human mesenchymal stem cells (hMSCs), and mouse dendritic cells (mDCs).
We found that VLPV PSA could infect mouse dendritic cells in vitro and induce a robust PSA-specific immune response in vivo.
The polymers were not toxic to mouse dendritic cells (DCs) and only displayed chain-length-dependent toxicity at a high concentration (1 mg/mL).
We use a set of time-course TSS data from mouse dendritic cells stimulated with lipopolysaccharide (LPS) to demonstrate the usefulness of our method.
A 2.6 kb 5' flanking region of mouse fascin-1 is sufficient to drive promoter reporter activity in mature mouse dendritic cells but not in immature cells; similarly, a 3.1 kb 5' flanking region of human fascin-1 specifically confers induction of reporters in mature human dendritic cells and other non-transformed fascin-positive cells.
Here again we see similarity in gene expressions between mouse dendritic cells.
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They all induce mouse dendritic cell maturation and B cell proliferation.
Hsp70-VP22268–301, Hsp70-VP22268 301n, was efficienthe taken up by mouse dendritic cell (DC) line DC2.4.
When added to DC2.4 cells, a mouse dendritic cell line, the fusion protein containing polyhistidine of 25 residues was efficiently taken up by the cells and efficiently distributed to the cytosol.
The BM8 monoclonal antibody (Affinity Purified anti-mouse F4/80 Antigen - Pan Macrophage Marker, eBioscience, San Diego, CA, USA) was used for macrophages and, the 33D1 monoclonal antibody (Affinity Purified anti-mouse Dendritic Cell Marker (33D1) eBioscience, San Diego, CA, USA), which recognizes a mouse dendritic cell-specific surface marker.
In addition to the examples described in the previous reviews [ 14, 15], extended site mechanisms for binding biantennary N-linked glycans have recently been demonstrated for the mouse dendritic cell immunoreceptor 2 (DCIR2) and BDCA-2 [ 9, 10].
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