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MS/MS data were searched against a Swissprot 51.6 mouse database using MASCOT 2.2 (Matrix Science, London, UK).
Data files were searched against the SwissProt mouse database using Mascot (http://www.matrixsciences.com) run on an in-house system, with a 10 p.p.m. mass accuracy for precursor ions, a 0.6 Da tolerance for fragment ions, and allowing for carbamidomethyl (C) as a fixed modification and for oxidation and dioxidation (M) as variable modifications.
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The chromosomal positions of thirty transposition events in offspring of seed mice from line 4563 were identified by querying the ENSEMBL online mouse genome database using the BLAST function.
Peptides from ICAT samples were identified by searching MS/MS spectra against the same mouse protein database using Sequest [24].
MS/MS spectra of peptides were analyzed against the mouse protein database using the Mascot version 2.3 search tool.
The BAC sequence was then compared to the mouse EST database using BLASTN, and multiple ESTs and their corresponding I.M.A.G.E.
The sequences were subjected to BLAST analysis against the annotated mouse genome database using Ensembl Genome Browser (release 45).
> -wrap-foot> The UCSC gene models are relatively conservative, so we searched the GenBank mouse EST database using BLAT (Kent, 2002) for the previously unreported junctions.
We carried out a similar search against the NCBI mouse EST database, using the Drosophila and human RNase III [ 18, 19] as query sequences.
In order to determine the coding sequence for the larger transcript, we searched the mouse EST database using each intron as well as the genomic sequence flanking the Mcoln1 gene.
Any PSG cDNA sequences that could still not be identified by this method were determined by BLAST analysis of the mouse genome database using known fragments of the sequence to be determined.
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