Twenty one days after intracranial implantation of tumors, mice were sacrificed and cryostat sections of brain were prepared.
Immunochemistry on cryostat sections through mouse embryos were performed as described in [32].
In contrast, Mabe and Hoover [ 18] counted neurons in serial cryostat sections of mouse atria.
We used an immunohistochemistry approach to identify the localization of Kir6.1 protein in cryostat sections of mouse ventricles.
Initially, we demonstrated the effectiveness of using confocal microscopy to image thick cryostat sections from mouse aorta stained with both histochemical and fluorescent dyes (Taatjes et al. 2000).
Immunohistochemistry was performed on cryostat sections of mouse liver as previously described (Patel et al, 2012) using anti-ATOH8 and fluorescein isothicyanate- conjugated secondary (Dako, Ely, UK).
Activities of NADPH- and NADH-producing dehydrogenases were visualized using metabolic mapping [ 53, 56] using 10 μm thick unfixed cryostat sections of mouse brains infiltrated with E478, E434 or E98 glioblastoma xenografts [ 41].
Staining of serial cryostat sections with mouse monoclonal anti-CD3 (BD Biosciences, San José, California, USA), anti-CD68 (marker of monocyte/macrophage lineage, KP-1 clone, Dako Cytomation, Glostrup, Denmark) and anti-CD163 (resident tissue macrophage marker, BerMac3 clone, Dako Cytomation) antibodies was performed using a standard protocol.
Transcripts were purified by standard RNA precipitation, and the pellets resuspended in 50 μl DEPC-treated H20. Adult (P60) C57BL/6 mouse brains were cryostat sectioned (20 μm) in the sagittal plane, and in situ hybridization was conducted as described previously [ 9, 10].
Paraformaldehyde-fixed cells or 10-μM cryostat sections from mice perfused with 4%% paraformaldehyde were washed with PBS twice and blocked with PBS containing 10 % goat serum, 1 % BSA, and 0.3 % TritionX-100.
