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The SVZ from YFP mice was co-cultivated in contact with wild type mouse cortex (see organotypic preparation above).
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Furthermore, the enhancement of Rd seen in GhrR KO mice was associated with significantly increased glucose uptake in 6 out of 8 tissues tested (WAT, BAT, gastrocnemius, soleus, diaphragm and cerebral cortex; see Figure 4).
For comparison of the cortex of the PFC with the retrosplenial cortex see Fig. 5a, b.
We isolated astrocytes from S89G-DMP1 adult mouse cortex and generated primary astrocyte cultures (Figs. 3A and S2).
Quantifying ChAT+ axon density in P30 mouse cortex.
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Similar results were obtained with several sections, and on sections of other brain areas such as the cortex and the Substantia Nigra reticulata, and on the cortex of 24 days old mice (see Figure S2– Figure S4).
Consistent with our in vivo imaging results, we detected an increased phosphorylation of Ser845 in the synaptoneurosome fraction obtained from the adult barrel cortex of NgR1-deficient mice compared with that of WT mice (see Supplementary Fig 4 F ).
Consistent with this interpretation, while spine turnover is increased, the total spine number is unchanged in the adult cortex and hippocampus of NgR1-deficient mice (see Supplementary Fig 4 I ) (Lee et al. 2008; Akbik et al. 2013).
Among all those selected miRNAs, miR-29a increased most significantly in P60 mice cortex (Table 1).
Protective Zuo et al. (2016) Male C57BL/6J mice Cortex tissue Cortex neuron MCAO Hyperglycemia enhances ischemia-induced mitochondrial dynamic imbalance towards fission.
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