In a second approach, we obtained primary cultures of cortical neurons from TCF-βgal transgenic mice, a colony designed to analyze the activation of the Wnt pathway in vivo [21].
A.M.C. designed the study, organized and maintained the mouse colony, performed biochemical and histologic analyses, analyzed the data and cowrote the paper; M.F.
PP was responsible for the breeding the mouse colony, performing cell injections, monitoring tumour growth, and assisted in designing protocols, performing the animal experiments, immunostaining tissue, and collecting images.
We thank Dr. Thomas Cooper for assistance with transgene design, Kyle Brimacombe, Natalie Vajda, and Kathryn LeMasters for help maintaining our mouse colony, Dr. Timothy Myshrall for assistance with evaluation of histological sections, and Dr. Clemencia Colmenares for helpful comments on the manuscript.
One concern regarding the methods and design is that the authors appear to have used a separate wild-type mouse colony and compared them to ephrin-a2 KO mice inbred via a backcrossed colony.
Mouse tissue was provided to Celera by the Jackson Laboratory of Bar Harbor, Me., a leading mouse colony.
The New Yorker, May 4 , 1963P. 36 To accommodate the considerable mouse colony in residence at the Rockefeller Institute, a scientist there devised a mouse house.
Nothing can so much incite a colleague's displeasure as sending a mouse with some pox that decimates the guest mouse colony.
"We have the biggest mouse colony in the United States," said Dr. Copeland, "and it takes a lot of money to run that".
