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Sequencing the 16S rRNA gene from the two mouse colonies revealed significant bacterial differences.
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A 2007 2008 survey of over 19% of all colonies revealed a total loss of 35.8%.
Sequence analysis of 13 colonies revealed that the recombination was correct in all tested colonies.
Images of stained MCF-7-VEC and MCF-7-MEK5 coloniesonies revealed distinct differences in cell and colony morphology.
Ziehl-Neelsen staining of samples from the colonies revealed acid-fast bacilli.
Analysis through DNA sequencing of isolated round 4 colonies revealed 17 unique sequences (Table 1 ).
The ratio of kanR/cmR colonies revealed the extent of gap repair.
The sequencing of randomly selected colonies revealed the insertion at pre-defined sites (data not shown).
Typical colonies revealing small Gram-negative coccobacilli, were further analyzed to obtain full identification and biotype.
Retrospective examination of banked tissue from a now defunct YFP-16 mouse colony with very high YFP expression levels (∼7 times greater than those found in our standard YFP-16 colony) revealed more severe morphological abnormalities, suggesting a dose-dependent effect (Figure 3).
The bacterial composition in the two mouse colonies was analysed by 16S rRNA gene sequencing, which revealed specific bacterial compositions in the two colonies.
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