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The study, published today in The Journal of Neuroscience, reveals that ex-dieters remained more sensitive to stress than nondieters and were more likely to eat large amounts of high-fat mouse chow when under pressure.
Three to five days prior to imaging mice were placed on a low manganese diet (Harlan, Indianapolis IN) to reduce autofluorescence caused by normal mouse chow when imaging.
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Lexicon's KO mice did not have an obesity phenotype when fed mouse chow or high fat diet (data not shown) but subcutaneous fat was not examined.
To test these hypotheses, Ryr1 AG/+ and Ryr1 +/+ mice were weaned onto 0.6% K+ (level of potassium in standard mouse chow), and four weeks later, when muscle weakness is already observed, they were given either 5.2% K+ diet or enalapril (0.02 mg/ml in drinking water) for an additional four weeks.
Mice were between 2 and 3 months of age when exposures began, and were fed an irradiated commercial mouse chow diet (LM-485; Harlan, Indianapolis, IN).
Because these mice continuously lost weight, we stopped PED feeding to switch back on standard chow, when weight loss was beyond 20% of their starting body weight (Fig. 4B).
There they were in several small cages, grouped with normal mice, all of them nibbling on mouse chow pellets.
There is all-you-can-eat mouse chow, free veterinary care and a guaranteed absence of feline harassment.
From a nutritional perspective, the feeding on mouse chow was begun during suckling.
They were acclimatized for 2 weeks on pelletized mouse chow before the commencement of the experiment.
Autoclaved water and sterile mouse chow were provided ad libitum.
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