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Four groups of five rats each were acclimatized on pelletized mouse chow for seven days, after which diabetes was induced by a single ip injection of alloxan in all groups except group 1, which served as control.
For recovery experiments mice were fed Dox for 4 weeks and then food was changed to normal mouse chow for 2 4 weeks to allow recovery of thymic architecture.
To investigate whether progenitor cells in the thymus were permanently lost or simply blocked in development when Wnt signaling was inhibited, TetO-Dkk1 mice were treated with Dox for 4 weeks and then Dox food was withdrawn and replaced with normal mouse chow for a 4 week chase.
While some mice were maintained on the dox diet, others were removed from the dox treatment by replacing the dox diet with regular mouse chow for 3 days.
28 Shortly, 5 6-month-old female C5 6-month-oldere feed with 0.3% w/w Cuprizone (biscyclohexane oxaldihydrazone, Sigma-Aldrich, St. Louis, MO) in a diet of ground mouse chow for a period of 10 weeks.
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The sham and the CLP animals were allowed to recover with free access to water and mice chow for 18 hours post-surgery prior to treatment with rHuEPO in the CLP group.
After ingesting the L. moncytogenes-contaminated bread, each mouse was returned to its original cage and was given unrestricted access to mouse chow and water for the duration of the experiment (5 days).
Celecoxib-treated mice and control mice, given standard mouse chow only, were fed for one week.
Therefore, to examine the role of Cox-2 in this model, mice were provided with standard mouse chow supplemented with celecoxib for one-week prior to treatment with AP20187 to activate iFGFR1.
Mice were fed NIH 31 mouse chow, which was assayed for estrogenic activity as previously described (Thigpen et al. 1999), and fresh water was provided in polycarbonate water bottles ad libitum.
Mice fed with control mouse chow were used as controls for each of these three test groups; these control mice were born from the same breeding population, housed contemporaneously, and euthanized in parallel with the corresponding test group.
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