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In summary, I stress that the conclusions of Levanon et al (2011) are not well supported because critical information, be it references or relevant KO mouse characteristics, was omitted.
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The mice characteristics are listed in Additional file 7: Table S4.
The mouse lemurs with divergent coat characteristics were included in the M. griseorufus clade, as they shared identical mtDNA haplotypes with individuals displaying the more typical M. griseorufus morphotype.
These characteristics are hallmarks of mouse liver adenoma with activated MAPK signaling; e.g. see [ 48].
When strains with these virulence characteristics were inoculated into mice, deaths exceeded 80% compared with mortality rates of <5% in mice inoculated with strains lacking these characteristics.
Five of the constructs with suitable characteristics were tested in mice as a single dose 5 × 1011 virions (vir) vaccine and were found to be able to stimulate production of GnRH-specific antibodies, but not to suppress testosterone (indirect indicator of GnRH antibody neutralizing properties).
In vivo biodistribution and pharmacokinetic characteristics were studied in A549 lung tumor-bearing Swiss Nu/Nu mice.
However, as shown in Figure 6D, Ca2+-dependent binding of annexin V is not reduced in macrophages from ABCA1−/− mice, indicating that this characteristic is not ABCA1-dependent.
For assessment of tumor micro-environmental characteristics, mice were injected intra-peritoneally (i.p).
These anti-NPM antibody-positive SLE sera were analysed by immunoblotting on HL-60 cell protein separated by 2D PAGE (Fig. 5a); most of them (80%) reacted consistently with a spot of molecular mass (36 kDa) and pI (4.5) that had the same coordinates as that recognized by male WB mice and was characteristic of NPM (Fig. 5c).
On the other end, the 10 mg/kg dose was very efficient on lung tumors but induced a drastic body weight loss, almost 15% at the end of the experiment, accompanied by a general posture of the mice which was characteristic of a high toxicity.
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