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The first clone (clone ID number CD00130 see CDT-DB [www.cdtdb.brain.riken.jp]; DDBJ/GenBank/EMBL accession number BP426385) was isolated by fluorescent differential display analysis of the mouse cerebellar transcriptome during postnatal developmental stages, as previously described [25].
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The present study identified a developmentally regulated transcript, PLD4, after searching the cerebellar development transcriptome database (CDT-DB) for characteristic spatiotemporal gene expression patterns during mouse cerebellar development [25].
Gao, W. et al. Optical imaging of long-term depression in the mouse cerebellar cortex in vivo.
Dunbar, R.L. et al. Imaging parallel fiber and climbing fiber responses and their short-term interactions in the mouse cerebellar cortex in vivo.
PBO inhibited Sonic Hedgehog ligand-induced Gli signaling and mouse cerebellar granular precursor cell proliferation.
ATXN1 insoluble aggregates were detected on Atxn1 154Q/+ mouse cerebellar soluble fractions 7 and 8.
Also, dyclonine treatment for 1 week increased FA-YG8 mouse cerebellar aconitase activity (Fig. 5B).
RNA was isolated from mouse cerebellar tissue and transformed to cDNA as previously described (see above).
Extrinsic signalling has previously been implicated in some aspects of mouse cerebellar development.
Methylmercury treatment also resulted in impaired mitochondrial dehydrogenase activity in cultured rat cerebellar granule cells [ 31] and mouse cerebellar neurons and astrocytes [ 32].
The analysis indicated the existence of ~25% novel transcripts in the developmental mouse lens transcriptome.
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