To conclude, the emerging picture from our labeling and functional studies is that granule cells and oligodendrocytes are major ET targets in the mouse cerebellar cortex.
The goal of this study was to acquire quantitative data on the organization of inhibitory synapses in the ML of the mouse cerebellar cortex.
The lack of ET-staining on the molecular layer and on GFAP-expressing cells in the granule layer of acute cerebellar slices or primary cultures from the mouse cerebellar cortex indicates that neither Bergman's radial glial cells nor cerebellar velimentous astrocytes are targeted by ET. This contradicts previous reports that GFP-tagged ET binds to astrocytes [25].
Under the conditions used to prepare primary cultures of mice cerebellar cortex, most of the cells present are granule cells (96%), few are astrocytes (<4%) and none are oligodendrocytes, Purkinje cells or fibroblasts.
While most brain regions, including the olfactory bulb, subventricular zone and the cerebral cortex, did not reveal any histological abnormalities in Txnrd1-NS null mice, the cerebellar cortex displayed striking morphological alterations.
Four to seven replicas were used for quantification of Kv4.2 immunolabeling in the following brain areas: ITCs, field CA1 of hippocampus (mouse, 1.4 2.0 mm caudal to Bregma; rat, 3.1 3.8 mm caudal to Bregma) and the cerebellar cortex (mouse, 6.0 6.6 mm caudal to Bregma; rat, 11.3 12.3 mm caudal to Bregma).
in situ hybridization revealed that the expression of NR2B transgene was exclusively observed in the cerebellar cortex of tg mice (Figure 1E), but not of their littermate control (thereafter control) mice (Figure 1D).
To investigate the mechanism responsible for the reduction of Purkinje cells in Hipk2 −/− mice, we performed immunofluorescence analysis on coronal cerebellar cortex sections from p120 wild-type and Hipk2 −/− mice.
(A ) Extracellular recordings were made from PCs in the cerebellar cortex of awake mice, using double barrel glass electrodes (right).
We imaged 101 sites on the surface of cerebellar cortex in 22 mice, including paravermal locations in lobules V, VI, and more lateral locations in simplex.
In Kv3.1 KO mice, GFAP staining significantly increased in cerebellar cortex, where Kv3.1 is normally highly expressed, but displayed in a patchy pattern in parts of the hippocampus.
