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To confirm that the mouse cells were cleared of prions, the researchers stopped treatment to see what would happen.
Normal mouse cells were defined as cells that did not stain for Ki67 or Bcl-2 and had morphologies of normal mouse fibroblasts, adipose, and lymphoid cells.
Mouse cells were subsequently incubated with streptavidin-PerCP for 20 min on ice.
The infected or uninfected mouse cells were processed for epifluorescence microscopy, TEM and SEM.
Thus, the transgenic mouse cells were more permissive than human cells for virus entry.
Expression data [8] for the three mouse cells were employed to stratify genes based on expression level.
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Expression of survivin following transfection in mouse cells was corroborated.
Traditional mouse ES cells, he explains, cannot tell us a great deal about human ones because mouse cells are "more primitive".
In the absence of Suv39h1/h2 H3K9me3 at pericentromeric heterochromatin of mouse cells is lost [51].
MethoCult 03434 (for mouse cells) was obtained from StemCell Technologies (Vancouver, BC, Canada).
We now conclude that HCMV infection in mouse cells was blocked before DNA replication.
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