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To identify proteins secreted by senescent mouse cells, we used primary fibroblasts from adult mammary glands (mBFs) or embryos (MEFs).
To test whether also AtCRT3 can complement the CRT-deficient mouse cells we expressed the AtCRT3 fused to a HA-tag in these cells.
Using different human and mouse cells, we detected the same unique staining pattern with two different antibodies raised against distinct Dicer peptides (Fig. 1).
Using mouse cells, we previously found that the signaling pathway induced by Plasmodium is independent of MyD88 (and likely of TLR) and it is different from the pathway used by crystallized uric acid to trigger IL-1β [7].
First, if we are able to fully document those factors that play a role in the enhanced osteogenesis observed in human as compared to mouse cells, we may further capitalize on them via gene or protein manipulation.
To comparatively investigate the roles of ND10 and ND10 proteins in cross-species infections (MCMV in human cells and HCMV in mouse cells), we performed an immunofluorescence assay to detect the effects of CMV infection on ND10.
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"The process was very similar to the one we used on mouse cells, but we used human dermal fibroblasts that we purchased commercially," Dr Vacanti said.
For electroporated hindbrains in which mouse Hoxb4- cells locally outnumber mouse Hoxb4+ cells, we observed strong apical N-cadherin staining in the centres of mouse Hoxb4+ cell clusters, correlating with increased apical constriction and neuroepithelial curvature (Fig. 5D-F).
To test the specificity of the anti-hCAP-H antibody in mouse meiotic cells we performed an immunoblot analysis with extracts of mouse testis, using extracts of HeLa cultured cells as a control.
We will have a better understanding of infectivity of particles released from mouse cells once we have improved the transcription and processing of HIV-1 Gag protein.
Following our initial observations in mouse EC cells, we then examined the phenotypic consequences of depleting geminin, Emi1, or Oct4 in B6/Blu-1 mousESES cells.
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