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A total of 130 gene expression data sets generated on a single microarray platform (Affymetrix MOE430-2.0) were collected representing 60 different primary mouse cells, tissues and cell lines (Supplementary Table S1).
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DNA (cytosine-5 -methyltransferases cytosine-5 -methyltransferases
Yoder, J. A., Soman, N. S., Verdine, G. L. & Bestor, T. H. DNA (cytosine-5 -methyltransferases cytosine-5 -methyltransferases
Total RNA was isolated from MSCs, human and mouse cells and tissues by the guanidium-thyocyanate method using Trizol (Gibco, Invitrogen) followed by phenol extraction.
PolgA is the target of DNA methylation in mouse cells and tissues.
The same changes were triggered by telomere dysfunction in mouse cells and tissues including the intestinal crypt epithelium.
We also added data from a previous microarray analysis from numerous mouse cells and tissues to further illustrate the expression patterns for Esrp1 and Esrp2.
Recently, Feng et al. [ 20] have made similar observations by comparing p53 response to γ and UV radiation in young and aging mouse cells and tissues.
Using a meta-analysis approach, we identified a transcriptional signature that distinguished the FAE from a large collection of mouse cells and tissues.
Importantly, several previous studies have shown p53-responsive expression of TIGAR in mouse cells and tissues such as the liver and heart, and p53 binding to the Tigar promoter was also detected in the liver.
Here, we used a meta-analysis approach to identify a co-expressed transcriptional signature that distinguished the FAE from a large collection of mouse primary cells, tissues and cell lines.
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