Previous work in cultured mouse cells indicated that rRNA is regulated by methylation of a single CpG dinucleotide at position −133 residing at the upstream control element (UCE) [31].
The data we obtained from mouse cells indicated that the defect in cohesion is specific to the centromere; therefore, we tested whether BAF180 is also important for mediating cohesion between telomeres.
Studies have shown that p73 can regulate neural stem cell maintenance (Agostini et al., 2010), and the overexpression of transactivation-deficient p73 proteins resulted in the proliferation of human and mouse tumor cells, indicating oncogenic activity of truncated p73 isoforms (Stiewe et al., 2002).
Although THOC5 is detected mainly in the nucleus in NIH3T3 and Hela cells, heterokaryon assay experiment reveals that THOC5 is able to shuttle from human Hela cells to mouse NIH3T3 cells, indicating that THOC5 is nuclear/cytoplasmic shuttling protein.
(C ) A heat map illustrating ChIP-seq signal at all CGI transcription start sites (TSSs) in mouse ESC cells indicating depletion of KDM2A and enrichment of KDM2B at polycomb target CGIs marked by RING1B and EZH2.
The indirect cytotoxicity assessments demonstrated the photocrosslinked hydrogels was compatible to mouse fibroblasts (L929 cells), indicating their potential as tissue engineering scaffolds.
Similar results were obtained with cDNA isolated from mouse circumvallate taste cells, indicating the presence of a GABAB heterodimer in peripheral taste cells (see Figure 5).
Most importantly, EGFP-tagged pc/glis3 protein localized in primary cilia as well as in the nucleus when expressed in mouse renal epithelial cells, indicating a strong connection between pc/glis3 and ciliary function.
Indeed, siRNA mediated knockdown of MSK1 as well as its dominant-negative mutant suppressed TPA and EGF induced transformation of JB6 mouse epidermal skin cells, indicating a crucial role of MSK1 in tumorigenesis [48].
Elevations of phospho-S6 at S235/236 were also observed in the stable transfectants of shRNA p27 generated from MEFs of different backgrounds and gestational ages, as well as from mouse epidermal Cl41 cells, indicating that the inhibitory effect of p27 on arsenite-induced S6 phosphorylation at S235/236 was prevalent, which was neither cell type-specific nor developmental stage-dependent.
