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We were able to infect mouse cells expressing human CD81 and occludin (OCLN the minimal set of entry factor factors required for HCV uptake into mouse cells.
To assure that this is the case we introduced these amino acids, essential for ERp57 interaction (Asp and Glu), to the positions of Asn268 and Leu270 in AtCRT3, respectively, and then generated five transgenic mouse cells expressing this AtCRT3 variant, referred to as AtCRT3m.
To test whether plant CRTs, i.e. the AtCRT3 and CRT1a [11], undergo N-linked glycosylation when expressed in the mouse cells we treated lysates derived from mouse cells, expressing either AtCRT1a or CRT3, with the glycosidase PNGase F that removes N-linked glycans (Figure S2A).
In the RCAS/Tv-a glioma mouse modeling system, avian retroviruses (produced from RCAS plasmids) infect mouse cells expressing Tv-a (the receptor for RCAS viruses).
TC-1 cells are derived from immortalized mouse cells expressing HPV16 E6/E7 and Ras oncogenes and are able to produce tumours in syngeneic C57BL6 mice [ 21].
PDGF βR is constitutively activated in transformed mouse cells expressing BPV-1 E5, and binding of BPV-1 E5 to PDGF βR induces its activation in mortal human fibroblasts [ 18, 22].
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Because the factors secreted by SEN XRA) mouse cells overlapped substantially with the human SASP, we conclude that mouse cells express a human-like SASP, at least when induced to senesce at a physiological O2 concentration (Fig. 1B; see Fig. S1A C for arrays, significance and comparisons).
We found that human, but not mouse, cells express the signature and Slug is the only upregulated EMT-inducing transcription factor.
However, following M.tb infection BMDM from C3HeB/FeJ mice, cells expressed significantly weaker cytokine responses than BALB/c mice (Fig. 2B).
One unit of FasL was defined as the dilution that gives a half-maximum response with W3 cells, mouse WR19L cells expressing mouse Fas (Watanabe-Fukunaga et al., 1992).
Fucosyltransferases-transduced mouse PCa cells expressing luciferase were inoculated into mice via left ventricle to compare the capability of bone metastasis.
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