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We thus studied the human SupT1 T-cell line with intensified RNAi developed by Llano et al. [37], and mouse cells containing homozygous gene trap mutations at the LEDGF/p75 locus developed by Sutherland and coworkers [42].
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The team found that the mouse cells contained resveratrol -- the original form of the compound.
We noted that mouse cells contain more 53BP1 foci than human cells at baseline (labeled as control in Figure 3C), suggesting that mouse cells are more likely to harbor unresolved DNA lesions than human cells.
In contrast, when cells were damaged in S phase then released, 8-10% of mouse cells contained micronuclei by three days, while less than 1% of human cells contained micronuclei.
Resistance to oxidative stress, is also potentially consistent with an altered but semifunctional MMR system since msh2−/− (mut S homolog 2) mouse cells contain increased levels of oxidative DNA damage (DeWeese et al, 1998) and MMR-deficient cells can be somewhat resistant to H2O2 (Glaab et al, 2001).
Inactivation occurs early in development, thus much of our knowledge regarding the initial events has been derived from studies utilizing mouse embryos or differentiating mouse ES cells containing Xist transgenes or mutations (reviewed in [1]).
Over 10,000 mouse strains and more than 20,000 mouse ES cells containing gene-trapped or targeted mutations are available to researchers.
Mouse spleen cells (containing 5 × 10 OT-II or DO11.10 cells per mouse) or further sorted populations, as mentioned wherever appropriate, suspended in normal saline, were transferred into anesthetized recipient mice i.v. Cells were parked in the recipient mice for various time periods as mentioned.
Mouse ES cells containing the mutated Rosa26 promoter regions were microinjected into blastocysts at 3.5 days post-coitum (dpc) obtained from natural matings of C57BL/6J mice.
Mouse NS cells containing a constitutively expressed marker for green fluorescent protein (GFP) were used to investigate the process of neural differentiation.
(B ) Mouse ES cells containing a MN-specific GFP marker were used to isolate GFP expressing (GFP+) MN cells and to monitor induction of MN under different sonic hedgehog (SHH) concentrations.
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