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Higher scores indicate that the transcriptional signature of the mouse cell subpopulation is found in the target sample.
At least three biological replicates of RNA from each human and mouse cell subpopulation were profiled for miRNA expression using a Taqman array system (Applied Biosystems, MA, USA).
For each target sample (mouse tumor or normal mouse mammary cell subpopulation), a set of signature scores was computed to measure the transcriptional activity of each mouse cell subpopulation in that sample, by using a method previously described [ 7].
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The four mouse mammary cell subpopulations exhibited distinct gene signatures.
RNA was prepared from freshly sorted mouse mammary cell subpopulations (mammary stem cell (MaSC -enriched, coMaSC -enrichedl progenitor, mature luminal and stromal committed used for gene expression profiluminalalysis on the Illumina progenitor
Smyth GK, Wu D, Lim E, Visvader JE, Lindeman GJ, 2010, Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers, GSE19446 ; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSE19446, In the public domain at GEO: http://www.ncbi.nlm.nih.gov/geo/.nih.gov/geo/
Smyth GK, Wu D, Asselin-Labat M, Lindeman GJ, Visvader JE, 2010, Mouse mammary epithelial cell subpopulations from pregnant and virgin mice, GSE20402 ; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSE20402, In the public domain at GEO: http://www.ncbi.nlm.nih.gov/geo/.nih.gov/geo/
In naïve mice the CCR6+ Th cell subpopulation proliferated vigorously.
After being sorted by anti-CD15-SPIONs, the obtained cell subpopulation from mouse SVZ could self-renew and generate numerous neurospheres after 72 h of culturing).
We have previously used the markers 33A10, CD24 and Sca-1 to identify mouse mammary epithelial cell subpopulations.
To obtain a broader picture of the molecular characteristics of the different mouse mammary epithelial cell subpopulations, gene expression profiles of these cells were obtained.
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