Consistent with this speculation, we found that human-cell ND10 proteins, such as SP100, PML, and Daxx, strongly suppress MCMV viral gene expression (Fig. 6), and mouse cell ND10 protein also represses HCMV gene expression (Fig. 7).
The function of the YDG/SRA domain motif (PF02182) is unknown, but is found in a novel mouse cell proliferation protein Np95, in which the domain is important both for the interaction with histones and for chromatin binding in vivo [60].
In addition, we report the proteomics analyses of complex biological samples using RP-HILIC with mouse chondrogenic cell protein extracts and deglycosylated human serum.
A total of 3,432 proteins were identified (1,758 for ES; 2,391 for TS; 2,442 for XEN; 2,169 for EpiSC; see Table S1 available online), which represents one of the largest mouse stem cell protein data sets reported (Van Hoof et al., 2006; Wang et al., 2008a).
Values for each gene were normalized to expression levels of β-actin (mouse cell lines) or ribosomal protein S14 (human cell lines).
For example, a recent study (Spruijt et al., 2013) employed a generic methylated DNA probe to pull down potential methylated DNA-binding proteins in mouse cell lysates, resulted in the identification of 19 proteins.
Twelve hours later, the mice were sacrificed and whole cell protein extracts or total RNA were prepared from the FB region (containing the cerebrum, thalamus, hypothalamus and limbic system).
In a previous report, we performed proteomic analysis of primary cilia from mouse cells and identified 195 proteins as candidate cilia proteins (Ishikawa et al., 2012).
When the team genetically altered mouse cells to overexpress a protein called Wnt-1, the modified cells ignored cues that normally prompt them to develop fat globules.
These data are in contrast to the mouse cells studied, where Gli1 protein cannot be detected without challenging the cells with Shh.
