The deletions of either LCD1 or LCD2 in mouse Lats2 abolished its tumor suppressor activity in immortalized mouse cell line (Visser and Yang, 2010).
To test whether trehalose could improve stress responses of higher eukaryotes, a mouse cell line was genetically engineered to express bacterial trehalose synthase genes.
By including only unique reads with more than 50 reads counts, we predicted 8 novel candidate miRNAs in human cell lines and 2 in the mouse cell line (Table S6).
The effects were not restricted to human cells, as overexpression of mouse RNASET2 in a mouse cell line TM6 also led to an increase of both in organello mtRNA synthesis and degradation rates (Fig. S3A C).
These results reveal for the first time that human adenovirus type 5 (Ad5 -based oncolytic virus cAd5 -basedied toncolyticompetent mouse with the introduction of CAR and E1B-55K to syngenic mouse cell line.
To systematically search for miRNAs that are modulated by TDP-43, we performed RNA interference (siRNA) mediated TDP-43 knockdown in both human and mouse cells, specifically two human cell lines (neuroblastoma SH-SY5Y and glioblastoma SNB-19) and a mouse cell line with neuronal features, HT22 (see Fig. S1).
These observations suggested that ACCNS cells were a mouse cell line.
To this end, we utilized a transgenic mouse cell line containing the JCV early promoter expressing the early genome.
The mouse cell line Hepa1 and human cell line Hela were transfected with miR-127 expression vector.
Genomic DNA of a mouse cell line containing a single copy of the neo gene was used as a positive control to examine the sensitivity of the detection.
A similar staining pattern was noted in the RT4 transitional carcinoma cell line (Figure 5b), supporting the epithelial nature of the derived mouse cell line.
