Mouse monoclonal α-GFP primary antibody (catalog number: A-11120), rabbit polyclonal Gαi1/2 (catalog number: 371723) and Gαo/i (catalog number: 371726) primary antibodies and the secondary antibodies (mouse – catalog number: A00160 and rabbit – catalog number: A00098) were purchased from Molecular Probes (Eugene, OR, USA), Calbiochem (Billerica, MA, USA) and Genscript (NJ, USA), respectively.
Enzalutamide was mixed with ground mouse chow (catalog number AIN-76; Research Diets Inc., New Brunswick, NJ, USA) at 0.43 mg/g chow.
The low-fat (regular) mouse chow (catalog no. 2018; 3.1 kcal/g; 24% kcal protein, 58% kcal carbohydrates, 18% kcal fat) and the high-fat (Western) mouse chow (catalog no. TD.88137; 4.5 kcal/g; 15% kcal protein, 43% kcal carbohydrates, 42% kcal fat) were purchased from Harlan Laboratories (Madison, WI).
We also assessed them in relation to the mouse imprinting catalog (http://www.mousebook.org/catalog.php catalog=imprinting). Interestingly, 8/32 (25%) of identified miRNA transcripts in the voluntary consumption treatment paradigm, 2/24 (8%) in the T1 paradigm, 13/45 (29%) in the T2 paradigm and 13/68 (19%) in the T3 paradigm mapped to three known imprinted regions of the mouse genome.
Biotinylated goat anti-mouse IgG (catalog no. M30215), goat anti-mouse IgM (catalog no. M31515), and FITC-conjugated streptavidin (SA1001) were purchased from Caltag Laboratories (Burlingame, CA).
Mouse Mills catalogs, for ordering dolls clothes made by mice, who take buttons for pay.
After wash with PBS, immunogold conjugated goat anti-mouse IgG (catalog no. EM.GMHL15, BBInternational) diluted at 1∶20 was dropped onto the membrane.
Anti-mouse CD45 (catalog no.
Secondary antibody detection was with a Cy3-labeled anti-mouse antibody (catalog no. 078-18-061; KPL).
Mouse recombinant VEGF165 (catalog # MGC70609) and VEGF121 (catalog # CYT-574) were purchased from ProSpec-Tany TechnoGene Ltd (Rehovot 76124, Israel).
Primary antibodies (Chemicon) were diluted to 1 800 (mouse anti-GAD67, catalog number MAB5406) and 1 500 (mouse anti-NeuN, catalog number MAB377).
