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It is a long and cumbersome procedure that involves combining pieces of DNA in embryonic stem cells and mouse breeding.
Then they checked the computer-predicted loci against published QTLs mapped through the conventional process of mouse breeding.
The authors are grateful to Minoru Tanaka and Sachiko Hirokawa for mouse breeding.
Mouse breeding and experimental protocols were subject to Institutional ethical approval and were performed under the terms of a U.K. Government Home Office licence.
Mouse breeding in the animal facilities of the Max-Planck Institute of Experimental Medicine and animal experiments including viral infections were performed according to European and German guidelines for the welfare of experimental animals.
We would like to thank K. Nakano and J. Horiuchi at NTT Software for supporting database construction, M. Kato and A. Itoh for assistance in programming, F. Hori for managing EST sequencing data, H. Monyer (Heidelberg Univ) for kindly providing pvalb-EGFP mouse breeding pairs, H. Uruma for secretarial assistance, and My Persson for English corrections.
This would take at least 18 months of additional mouse breeding.
Importantly, these experiments additionally confirm that rescue of Nmnat2gtE/gtE; Sarm1−/− axons is due to SARM1 deficiency and not other, undefined changes resulting from mouse breeding.
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The teams were led by David E. Harrison of the Jackson Laboratory, a mouse-breeding powerhouse in Bar Harbor, Me.; Richard A. Miller of the University of Michigan; and Randy Strong of the University of Texas Health Science Center.
"Antibiotic Delayed Aging in Experiments With Mice" (news article, July 9) may have left your readers with the impression that the Jackson Laboratory is only "a mouse-breeding powerhouse".
Academic Medicine Development Company, a nonprofit consortium of 28 medical schools, health centers and medical research institutions in New York, has announced plans to open a $10 million mouse-breeding laboratory in a 106-year-old, 116,000-square-foot commercial building next year in the former carpet mill area, now called i.park N-Valley.
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