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But the nature of this study speaks to another question: whether through fundamental alterations and improvements to mouse brains we could create sentient animals with levels of intelligence to rival our own – a concept known as "uplifting".
By transmitting US waveforms through hippocampal slice cultures and ex vivo mouse brains, we determined LILFU is capable of remotely and noninvasively exciting neurons and network activity.
Using homogenates of wild-type mouse brains, we observed that total mAPP as well as the corresponding phosphorylated form were largely recovered in fractions 5 9, where GM130 (Golgi marker protein, concentrated in fractions 7 9) and EEA1 (early endosomal protein, concentrated in fractions 4 8) were co-distributed (upper panel).
In order to evaluate human GBM progression in immunocompetent mouse brains, we performed MRI and histopathological analysis.
In mouse brains, we did not find any GFAP+ glial cell colabeling with spatacsin (Supplementary Material, Fig. S3D).
To determine the precise nature of the membrane surrounding aggregates in CHMP2BIntron5 mouse brains, we performed pre-embedding labelling electron microscopy.
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After we verify successful infusions of trypan blue with our microneedles into both 0.9% w/v agarose gel and sacrificed mouse brain, we demonstrate the possibility of drug infusions with not only precise targeting capability but also less brain damage by infusing dyes in vivo followed by immunohistochemistry (IHC).
In the adult mouse brain, we used an affinity chromatography approach, based on roscovitine immobilized on a solid matrix as previously described [9], [30].
To explore the expression pattern of Hdac7 in the mouse brain we performed immunohistochemistry to coronal sections of WT and R6/2 brains from animals at 14 weeks of age.
In mouse brain, we have shown that Rarb, Thrb and Stmn2 are significantly increased in prion-infected as compared to uninfected animals although for Stmn2 this effect was not replicated in RIIIS/J mice.
To visualize the presynaptic distribution in single neurons within the mouse brain, we designed a strategy in which a tripartite system was used to express fluorescently tagged synaptic proteins with both spatial and temporal control (Figure 1A).
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