Before treatment with tariquidar, Mdr1a/1b dKO mice showed higher brain uptake than WT mice throughout the entire PET scan (Figure 1).
As in the cell cultures, G-28 cell line derived tumors in mouse brain showed extensive uptake of QD-EGF (Fig. 2A,D) and no uptake of untargeted QDs.
Preliminary biodistribution studies (Table 1) with HPLC-purified 18F-7B inormalal mice show transient brain uptake values of 7.23 ± 0.47%ID/g and 1.55 ± 0.01%ID/g, 5- and 120-min post tail-vein injection, respectively, thus providing a 5 min/120 min clearance a ratio of 4.66.
Biodistribution studies of [18F]2 in ICR mice indicated high initial brain uptake and a relatively fast washout.
Brain uptake and access to mouse brain parenchyma, after systemic injection, were confirmed by gamma counting and capillary depletion, respectively.
In addition to the experiments with cultured cells expressing the transporters, we also examined CM and cocaine inhibition of dopamine uptake in mouse brain tissues.
The current study is aimed at bridging this knowledge-gap by a methodical investigation of Aβ40 uptake in mouse brain slices and rat primary hippocampal neurons as well as the internalization of Aβ40 and Aβ42 in neuron like PC12 cells.
The present BEI study demonstrated that forskolin enhanced [I]hAβ 1-40) elimination from mouse brain and the in vitro uptake study showed that forskolin increased [I]hAβ 1-40) internalization into TM-BBB4 cells, and this action was inhibited by MEK inhibitor.
Biodistribution studies in mice revealed comparably high initial brain uptake of [99mTc]5 and slow washout.
After CsA administration, the peak brain uptake in mice was at 1.5 min and at 1.2 min in rats.
