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Mouse brain tissues were kindly provided by Prof. Orly Reiner.
This is consistent with data on mouse brain tissues published previously [6], [7], [43].
Cells and mouse brain tissues were processed as previously described [1], [20].
The EST data (Fig. 6) although supporting high expression of MT3 in human and mouse brain tissues also reveal abundant expression in other tissues as well.
In addition to the experiments with cultured cells expressing the transporters, we also examined CM and cocaine inhibition of dopamine uptake in mouse brain tissues.
Total RNA was extracted from mouse brain tissues with TRIZOL (Invitrogen, Karlsruhe, Germany) and digested with DNase (amplification grade I; Invitrogen, Karlsruhe, Germany) following the manufacturers' instructions.
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However, immunohistochemical staining of 12C-irradiated mouse brain tissue showed 2 months later a marked inflammatory reaction in the hippocampus and a diffuse response in parenchyma.
Tang et al. used nonlinearity assisted iterative optimization (IMPACT) and focused light through mouse brain tissue and skull within ~5 s [15].
Finally, they tested their touch sensors and monitored whether the mouse brain tissue received the signals and fired in response.
Therefore, naloxone methiodide does have a lower affinity for opioid receptors than naloxone in mouse brain tissue, which must be taken into consideration in experimental designs.
The aim of this study was to assess the experimental and biological variations on this proteomic platform using mouse brain tissue.
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