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The effect of -crebanine on changes in 5-HT induced synaptic transmission was evaluated by electrophysiological recording of mouse brain slices (Jang et al. 2012).
Mouse brain slices exhibited discrete binding of nifrofam in the auditory cortex showing promise for examining cellular distribution of α4β2∗ nAChRs in brain regions.
In mouse brain slices, α was strongly location-dependent, ranging from 0.16 in thalamus to 0.22 in brainstem, and was sensitive to cell volume changes.
Binding of the higher affine [18F]NS14490 [22] in mouse brain slices correlated with the pattern of α7 nAChR expression and was displaced with the α7 nAChR-specific alkaloid methyllycaconitine [8].
In this study, we have imaged and monitored real-time release of neurotransmitter glutamate from mouse brain slices stimulated by nicotine and physiological salts using a newly developed two-dimensional (2D) imaging biosensor.
The selective interaction of arachidonic acid with not only artificial bilayer membranes but also with biomembranes provided the design of a new sensing system for the detection of arachidonic acid released in mouse brain slices.
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An angled, sagittal mouse brain slice preparation has been designed to facilitate concurrent stimulation of two major glutamatergic afferent pathways to the nucleus accumbens.
To more accurately reflect the situation in normal brain, the binding of these peptides to adult neurons was also determined in a mouse brain slice model.
Brains were quickly removed and cut in three 2-mm thick coronal sections using a mouse brain slice matrix (Harvard Apparatus, Holliston, MA, USA).
As a control, we tested glutamate application on a nucleated patch pulled from a neocortical pyramidal cell in an acute mouse brain slice.
After 24h mice (n = 5/group) were transcardially perfused with 4% PFA and brains were quickly removed and cut in 2-mm thick coronal sections using a mouse brain slice matrix (Harvard Apparatus, Holliston, MA, USA).
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