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In April , 2013 Deisseroth announced the new technology in Nature; the journal's Web site posted videos of a clarified mouse brain, showing a tangle of fantastically fine cells and nerve fibres, which glowed green against a black background.
(A ) Top, sagittal and coronal schematic views of a mouse brain showing cholinergic regions of interest.
(B ) Coronal section of the mouse brain showing the location of VMHdm (Allen Brain Atlas).
(C ) Left panel, section from 11-month-old p53-deficient mouse brain showing a rare GBM, higher magnification image on right.
(D ) In situ hybridisation (ISH) of NONCO7761 (3100003L05Rik ) at ZT6 in the mouse brain showing low yet distinct expression in the SCN.
(D ) Schematic of a forebrain cholinergic neuron in a dorsal view of the mouse brain showing the mediolateral cell body and arbor locations, the parameters displayed in panels (H ) and (I ).
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(G) The section of a non-injured mouse brain shows a dense perinuclear accumulation of mitochondria (left, arrowhead: nuclear membrane).
Confocal analysis of treated Npc1−/− mouse brain showed that many cholesterol negative neocortical neurons still exhibited ganglioside accumulation (Fig. 5C).
Founder #12 was found to possess the active transgene and β-gal staining of this mouse brain showed moderate transgene activity (data not shown).
As in the cell cultures, G-28 cell line derived tumors in mouse brain showed extensive uptake of QD-EGF (Fig. 2A,D) and no uptake of untargeted QDs.
The Hq mouse brain shows very few immunogold particles.
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