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Fig. 5 a Representative coronal S1R-stained WT and S1R-KO mouse brain sections.
S1R-KO mouse brain sections were found to be devoid of S1R staining.
Both antisera produced similar labelling patterns in mouse brain sections processed for immunofluorescence as described below.
The mouse brain sections used as reference space must be first selected and reconstructed.
The MSn analysis was performed directly on the hippocampus area of the mouse brain sections.
In the present study we determined the pattern of expression of Jarid1c in mouse brain sections by in situ hybridization.
Cellular expression of PLD4 mRNA in P7 and P21 mouse brain sections was analyzed by in situ hybridization (ISH) (Fig. 4).
Different attempts have been made to implement computer-assisted analysis for quantification of amyloid plaques on human and mouse brain sections [25], [46].
To determine the subcellular localization of chimeric mRNAs, non-radioactive in situ hybridization on transgenic mouse brain sections was carried out as previously described [14].
These binding studies were performed twice in mouse brain sections and once in human AD brain sections with the same results.
In situ zymography was carried out on E15 mouse brain sections (30 μm thickness) prepared with a cryostat (Reichert-Jung, LEICA).
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