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Forty-two N-glycans were analyzed from the mouse brain section.
The AAL lectin histostaining of an adjacent mouse brain section is shown in Figure 4A.
MS images at a pixel size of 100 μm were acquired from the mouse brain section.
In order to confirm the results of the staining, a normal mouse brain section was used as a positive control.
The resulting tryptic peptides in a mouse brain section were analyzed by mass spectrometry imaging at 50 μm pixel size.
The highest spatial resolution of a MALDI imaging experiment of tissue so far was obtained by analyzing a mouse brain section at 3 μm pixel size.
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Fig. 5 a Representative coronal S1R-stained WT and S1R-KO mouse brain sections.
S1R-KO mouse brain sections were found to be devoid of S1R staining.
Both antisera produced similar labelling patterns in mouse brain sections processed for immunofluorescence as described below.
The mouse brain sections used as reference space must be first selected and reconstructed.
The MSn analysis was performed directly on the hippocampus area of the mouse brain sections.
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