The same distribution pattern as in mouse brain was observed for Kv4.2 subunit labeling in rat brain, and both antibodies used (Kv4.2454 469, Fig. 2a c; Kv4.2209–225, Fig. 2d f) gave identical results.
Using homogenates of wild-type mouse brains, we observed that total mAPP as well as the corresponding phosphorylated form were largely recovered in fractions 5 9, where GM130 (Golgi marker protein, concentrated in fractions 7 9) and EEA1 (early endosomal protein, concentrated in fractions 4 8) were co-distributed (upper panel).
To further support the molecular link between amyloid β peptides and C. albicans cerebritis, we measured β and γ secretase levels from mouse brain homogenates and observed a significant increase in the protein levels of BACE-1 and PS1, which is a subunit of γ secretase (Supplementary Fig. 4).
While we did not detect most of these transcripts, all five minor 5' splice variants identified by us in MJD15.4 transgenic mouse brain were also observed in non-neuronal human PBLs.
Interestingly, increased expression and/or transcriptional activity in HD model mouse brain has been observed for other transcription factors reported to be affected by mutant huntingtin.
In a recent study focussing on QC expression in telencephalic and diencephalic mouse brain regions, we observed QC in a subpopulation of lateral and paraventricular hypothalamic neurons and in a moderate number of GABAergic interneurons in the hippocampal molecular layer, in the hilus of the dentate gyrus and in all layers of the neocortex [ 18].
In the mouse brain, CrT has been observed as early as embryonic day 13 [19], [19] and postnatal expression is observed in the hippocampus, neocortex, and basal ganglia [18].
A similar DRBP76 doublet in immunoblots of mouse brain lysates has been observed previously [25], but that study did not show comparison with cell lines.
In the adult mouse brain high levels were observed in the colliculi, cerebellum, medulla, olfactory bulb, and striatum (Fig. 2).
The indispensable role of Rnd3 in mouse brain development was also observed in two recent studies 9, 10.
