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Mouse brain microvascular endothelial cells (bEnd.3) were purchased from the American Type Culture Collection.
Herein we investigated the effects of ET-1 in COX-2 regulation in mouse brain microvascular endothelial (bEnd.3) cells.
We report imaging of the whole mouse brain microvascular system at resolutions sufficient to perform accurate morphometry.
The isolation and culture procedures of primary mouse brain microvascular endothelial cells (pMBMECs) have also been described in detail before [ 15- 17].
In this study, we investigated the molecular mechanisms underlying ET-1-induced COX-2 expression in mouse brain microvascular endothelial (bEnd.3) cells.
This allows us to use stains that label entire anatomical components, such as the mouse brain microvascular system, in time-scales more conducive to large-scale clinical research.
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One study showed that obesity exacerbates sepsis-induced inflammation and microvascular dysfunction in the mouse brain [ 26].
Orl-1, Orl-c, and E. coli DH5α harboring pOrl-1-CTX-M-1 were tested for invasiveness in human brain microvascular endothelial cells (10 ) and in a newborn mouse (C57BL/6 wild-type) model of meningitis (R. Mittal et al., unpub. data) to investigate the influence of CTX-M-1 production on virulence.
For example, brain microvascular defects were found to be significantly worse in genetically obese ob/ob mice [ 19].
Story No. 2: "Scientists Map Mouse Brain".
A mouse brain is the size of a peanut.
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