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Using a mouse brain matrix (Activational Systems, Warrren, MI), 1-mm thick slices were taken through the NAc or amygdala.
We removed the brains and cut 2 mm coronal sections with a mouse brain matrix.
The forebrain was divided into five coronal 2-mm sections using a mouse brain matrix (RBM-2000C; Activational Systems, Warren, MI, USA).
The brains were put in a 1 mm coronal mouse brain matrix (Ted Pella Inc, Redding, CA, USA) kept on ice and sliced.
Brains were removed and 1-mm tissue punches were prepared using 1-mm interval mouse brain matrix (Zivic Instruments, Pittsburgh, PA, USA) and 1-mm core diameter hollow needles.
Brains were removed and post-fixed in 10%% buffered formalin overnight, before being coronally dissected into three segments using an adult mouse brain matrix (RBM-2000C; ASI Instruments, MI, USA).
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Post-fixed tissues were rinsed in Leaching buffer (50 mM Tris, 150 mM NaCl, pH 8.0) and brains were cut in 2-mm-thick coronal slices using a mouse brain slice matrix (Harvard Apparatus, Holliston, MA).
Brains were quickly removed and cut in three 2-mm thick coronal sections using a mouse brain slice matrix (Harvard Apparatus, Holliston, MA, USA).
After 24h mice (n = 5/group) were transcardially perfused with 4% PFA and brains were quickly removed and cut in 2-mm thick coronal sections using a mouse brain slice matrix (Harvard Apparatus, Holliston, MA, USA).
The laser beam was rastered across the surface of the mouse brain tissue coated with matrix by manually moving the focusing lens perpendicular to the laser beam.
Survival of transplanted F3.Akt1 hNSCs in ICH mouse brain was identified by human nuclear matrix antigen (hNuMA)- or LacZ/β-gal-positive reaction.
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