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Experimental controls for ic and ip transmissions were inoculated under the same experimental conditions using identical quantities of uninfected mouse brain material.
To assess the ability of lysate derived from previously established cell lines to support PMCA, we prepared cell lysate in which PrPC was concentrated to the same level as wild type mouse brain material (Fig. 1A).
MG provided mouse brain material and performed standardization experiments for immunostaining.
Use of the vaccine has been limited because of inadequate supply due to the difficult production process, cost of production, a difficult administration schedule, and, probably most important, concern about side effects resulting from mouse brain material remaining in the vaccine.
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It should be noted that our in situ hybridization analysis revealed no reduction in cortical p39 expression in 12-week-old R6/2 mouse brain (Supplementary Material, Fig. S13).
To test this hypothesis, we inoculated strain-typing panels of wild-type mice with brain material from human vCJD case-patients from France, the Netherlands, Italy, and the United States.
Both hypoglycosylated and IIH6-positive intact α-DG proteins were detected in Hp/Hp and Hp/− mouse brains (Supplementary Material, Fig. S2).
This problem was overcome in this study by using HD model mouse brain as a material and by performing the screening using new strategy in which alterations in DNA binding of transcription factors were comprehensively analyzed by Protein DNA array.
In particular, different groups generated MECP2-mutated iPS lines and characterized the resulting neurons reporting morphological and electrophysiological defects corresponding to those found in both mouse brain and patient autoptic material.
For homogenization of brain material, mice were sacrificed by CO2 inhalation and their brains removed.
Small deposits of prion protein accumulated in the brains of inoculated mice after challenge with brain material from VV VPSPr patients.
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CEO of Professional Science Editing for Scientists @ prosciediting.com