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We mapped a real RNA-seq dataset (>200M 25 bp reads) from mouse brain, liver and muscle [13] to both Exon Spliced Junction (ESJ, see Methods) and, as a negative control, Exon Random Junction (ERJ, see Methods) databases using SOAP (v1.11) with up to 2 mismatches allowed [14].
When applied to a large dataset (>200M reads) consisting of mouse brain, liver and muscle mRNA sequences, and using independent transcripts databases as positive control, our method was proved to be considerably more accurate than previous ones, especially for detecting junctions originated from low-abundance transcripts.
Genomic DNA tissue samples from mouse brain, liver, kidney, heart, and lung were purchased from BioChain.
RNA-seq data for adult mouse brain, liver and muscle tissues were obtained from NCBI Sequence Read Archive SRA001030.
The ratio of donor mtDNA in cloned offspring reveals tissue-specific distribution in mouse brain, liver, and tail [ 89].
Assay efficiencies were calculated using five four-fold serial dilutions of a pool of mouse brain, liver and testis (BLT) cDNAs as described (36).
Similar(47)
We performed a genome-wide expression profiling in four different tissues of adult XX and XO mice (brain, liver, kidney and muscle), using the Illumina Mouse WG-6 v1_1 Expression BeadChip, which covers more than 45,000 transcripts and includes 1,326 probes interrogating the X chromosome transcriptome.
In situ hybridization on P20 fresh frozen mouse brain and liver sections was performed as described [15] with a few modifications, omitting the treatment with Proteinase K.
Ten genes were analyzed by in situ hybridization on P20 fresh frozen mouse brain and liver sections: Pecam (SmaI, T7), Cldn5 (XhoI, T3), Apcdd1 (HindIII, T7), Abcb1a (EcoRV, T7), Slco1c1 (XhoI, T3), Slco1a4 (XhoI, T3), Slc22a8 (XhoI, T3), Slc7a5 (SalI, T3), Itih5 (SalI, T3), Foxq1 (EcoRV, T7).
DNA was isolated from adult C57/Blk6 mouse brain and liver using Gentra Puregene Tissue Kit (Qiagen).
We observed differentially expressed patterns of miRNAs in mouse brain and liver.
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