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We aimed to investigate this by using [3H]DAMGO, [3H]DPDPE and [3H]U-69,593 to compare the ability of naloxone and naloxone methiodide to displace binding to μ, δ and κ opioid receptors in mouse brain homogenates.
Next, the effects of rifampicin on oxidative stress [lipid peroxidation in mouse brain homogenates and free radical-scavenging activity against diphenyl-p-picrylhydrazyl (DPPH)] were evaluated to clarify the underlying mechanism.
Mouse brain homogenates were fractionated as described [19].
We used mouse brain homogenates as a positive control for the anti-TRPM8.
Using differential centrifugation, we isolated synaptosomes from mouse brain homogenates, as described [14], [15].
Mouse brain homogenates were prepared in PBS, 0.05% sodium deoxycholate, and 0.05% Nonidet P-40.
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To make normal mouse brain homogenate, two frozen mouse brains (Harlan Sprague Dawley, Inc., Indianapolis, IN) were Potter homogenized in 10 ml of ice-cold PBS (phosphate-buffered saline without calcium or magnesium) containing Complete protease inhibitors (Roche, Indianapolis, IN).
MC3 was used to digest BSE301V infectious mouse brain homogenate (iMBH).
Moreover, the utility of the probe in imaging HOCl/ClO− was demonstrated in in vitro phantom imaging studies using mouse brain homogenate as biological relevant media.
We performed serial tenfold dilutions in cell culture medium containing healthy mouse brain homogenate.
However, reliable PEA quantification required only 3 mg of yeast homogenate sample but 30 mg of mouse brain homogenate [35].
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