Sentence examples for mouse brain extracts from inspiring English sources

We identified c-Jun amino-terminal kinase 3 (JNK3) as a binding partner of β-arrestin 2 using a yeast two-hybrid screen and by coimmunoprecipitation from mouse brain extracts or cotransfected COS-7 cells.

We observed the in vivo binding of AATYK1A to Cdk5/p35 using anti-AATYK1 coimmunoprecipitation from HEK293 cell extracts and mouse brain extracts.

We previously demonstrated that cdr2 and c-myc co-immunoprecipitated in mouse brain extracts, as well as in tissue culture cells [11].

To determine whether the endogenous Tmub1/HOPS is localized in the membranous compartment, we fractionated mouse brain extracts into cytosolic and membranous fractions (Figure 2F).

Consistently with the in silico expectation that Tmub1/HOPS possesses transmembrane domains (Figure 1A), endogenous Tmub1/HOPS was found in the membranous fraction of the mouse brain extracts (Figure 2F).

This antibody recognized a single polypeptide around 24 kDa in Western blots of mouse brain extracts (12).

The anti-pS34 antibody recognized AATYK1 immunoprecipitated from a mouse brain extract at a molecular weight ∼200 KDa (Fig. 5A, lane 1).

The possibility that lipin-1α stimulates the transcriptional activity of MEF2 by a direct coactivator mechanism is supported by our finding that lipin-1α coimmunoprecipitated with endogenous MEF2C in mouse brain extract (Fig. S4A) and with ectopically expressed MEF2C in HEK293A cells (Fig. S4B).

Mouse brain extract in SDS/PAGE loading buffer was obtained from Sigma Aldrich.

A higher protein concentration (40 μg/μL) of WT mouse brain extract decreased the adhesion of cells by approximately 75%.

(Bottom) GST pulldowns from a mouse brain extract using GST alone or GST fusions of OCRL as baits.