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To increase the physiological relevance, patient-derived GBM cells and mouse brain endothelial cells were used as model cell types.
We studied whether the cEND mouse brain endothelial cells can be suitable for in vitro safety assessment of infusion sets.
Cyclosporine increased the permeability of sodium-fluorescein and the cellular accumulation of rhodamine 123, a substrate of P-glycoprotein, in mouse brain endothelial (MBEC4) cells.
This study was designed to provide insight into the effects of JP05, a water extract of 12 herbs, on the levels of regulators in bEnd.3 mouse brain endothelial cells.
The increased CD31 correlates with an increased ability of the lin BMCs to adhere to both fibronectin and mouse brain endothelial cells.
To confirm MF1 inhibitory activity, we incubated mouse brain endothelial cells (bEnd.3 cells) in serum free condition overnight.
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To undertake this project, we first used a retroviral overexpresson strategy to drive LCN7 protein expression in mouse brain microcapillary endothelial (MB114) cells [14] and tumor derived SVEC4-10 (SVendotheliallial cells [15].
Primary mouse brain microvascular endothelial cells (mBVECs) were purchased from Cell Biologics and maintained in Complete Endothelial Cell Medium (Cell Biologics) according to the manufacturer's instructions.
To examine the effect of estrogen on NO release in cerebral endothelial cells, we used the immortalized TM-BBB cell line, which is derived from mouse brain vascular endothelial cells [28] (See material and methods).
In this study, we initially showed that transport of LIF is mediated by its specific receptor LIFRα (gp190), using both adult mice and monolayers of mouse brain microvessel endothelial cells.
The conditionally immortalized mouse brain capillary endothelial cell line (TM-BBB) was performed as previously described [61].
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